K9799-90A
Kynureninase (L-kynurenine Hydrolase, KYNU)
Size
£780.00
- SKU:
- K9799-90A
- Application:
- WB
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to aa1-465 from human Kynureninase expressed in Sf21 cell line.
- Clone:
- 12K123
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Kynureninase (KYNU) catalyzes the hydrolytic cleavage of the aa's L-kynurenine and L-3-hydroxykynurenine to give either anthranilic acid or 3-hydroxyanthranilic acid and alanine. KYNU and other "kynurenine pathway" enzymes degrade dietary tryptophan in the liver and are involved in the de novo biosynthesis of NAD+. KYNU and other pathway proteins in immune system cells, such as macrophages and microglia, catalyze inflammatory quinolinic acid (QA) production, which may cause neuronal damage in AIDS-related dementia complex, Alzheimer's, stroke, epilepsy, and Huntington's disease. Human KYNU shares 83% and 85% aa identity with mouse and human KYNU, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice