K1893-18C
KLRG1 (Killer Cell Lectin-like Receptor G1, MAFA, 2F1-Ag) (PE)
Size
£499.00
- SKU:
- K1893-18C
- Application:
- Flow Cytometry
- Purity:
- Purified
- Storage Conditions:
- 4°C Do not freeze
- Supplier:
- United States Biological
- Host:
- Hamster
- Immunogen:
- Activated NK (A-LAK) cells from B6 (H-2b) mice
- Clone:
- 5K32 (2F1)
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. labeled with R-Phycoerythrin (PE).
- Extra Details:
- Killer cell lectin-like receptor G1 (KLRG1; formerly known as mouse MAFA or 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family that contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. The 2F1 antibody stains 30-60% of NK1.1+CD3- splenocytes, and a small fraction of T cells in all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL). Cell surface expression of KLRG1 is up-regulated by expression of MHC class I molecules. The effect of MHC class I expression is indirect, and can be mediated by interactions with class I-specific Ly49 inhibitory receptors. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: ≤0.2ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice