K1891-52D
KLF10 (Krueppel-like Factor 10, EGR-alpha, Transforming Growth Factor-beta-inducible Early Growth Response Protein 1, TGFB-inducible Early Growth Response Protein 1, TIEG-1, TIEG, TIEG1)
Size
£843.00
- SKU:
- K1891-52D
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to Asn31-Ser154 from human KLF10 expressed in E. coli.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- LF10 is a 55-65kD member of the Sp1 C2H2-type zinc-finger protein family. It appears to be very widely expressed, and functions as a transcriptional repressor in a noncovalent complex with JARID1B. KLF10 is induced by TGF-B, GDNF and estrogen, and inhibits transcription of genes such as BI-1, OPG and SMAD7. By suppressing SMAD7, it promotes TGF-B signaling. Human KLF10 is 480aa in length and contains three C2H2-type zinc-finger motifs aa369-451. There is one potential alternate splice form that shows a deletion of aa2-11. Over aa31-154, human KLF10 shares 79% aa identity with mouse KLF10/mGIF. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml with lysates of SH-SY5Y human neuroblastoma cell line and IMR-32 human neuroblastoma cell line and cytoplasmic extracts of K562 human chronic myelogenous leukemia cell line under reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice