K1891-20C
Klebsiella sp. (FITC)
Size
£512.00
- SKU:
- K1891-20C
- Application:
- IF
- Purity:
- Purified IgG fraction to a purity of > 95%.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Klebsiella pneumoniae
- Format:
- Supplied as a liquid in PBS, pH 7.2, BSA (10mg/ml), 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- Members of the Klebsiella genus typically express 2 types of antigens on their cell surface. The first, O antigen, is a component of the lipopolysaccharide (LPS), of which 9 varieties exist. The second is K antigen, a capsular polysaccharide with more than 80 varieties. Both contribute to pathogenicity and form the basis for serogrouping. Applications: Suitable for use in Immunofluoresence. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:10-1:50. Direct FA staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining. Optimal dilutions to be determined by the researcher. Titer: >1:1000 by indirect Immunofluorescence Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice