K1851
Kip2, p57 (cdk Inhibitor, Cyclin Dependent Kinase Inhibitor)
Size
£793.00
- SKU:
- K1851
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa303-316 (C)GVGSVEQTPRKRLR of human Kip2 (KLH). Species sequence homology: mouse-14/15.
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
- Extra Details:
- The eukaryotic cell cycle is driven by the sequential action of a family of serine/threonine kinases, the cyclin dependent kinases (cdks) acting in concert with their regulatory subunits, the cyclins. p57 Kip2 is a potent inhibitor of several G1 cyclin/cdk2 complexes but unlike p27 Kip1 is not regulated by p53. Kip2 is thought to be involved in decisions to exit the cell cycle during development and differentiation. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml detects Kip2(~57kD) in a HeLa nuclear extract and a bovine brain nuclear extract. Immunoprecipitation: 5ug immunoprecipitates Kip2 from a HeLa nuclear extract and a bovine brain nuclear extract. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb Positive Antigen Control Included: H1838: HeLa Nuclear Extract Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice