K1851-01
Kip2, p57 (cdk Inhibitor, Cyclin Dependent Kinase Inhibitor)
Size
£895.00
- SKU:
- K1851-01
- Application:
- IHC-Fr
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human p57Kip2. Cellular Localization: Nuclear.
- Clone:
- 3F244
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
- Extra Details:
- p57Kip2 (or CDKN1C) is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. The gene encoding human p57Kip2 is located on chromosome 11p15.5, a region implicated in both sporadic cancers, Wilm’s tumor, and Beckwith-Wiedemann syndrome (BWS), a cancer syndrome, making it a tumor suppressor candidate. BWS is characterized by numerous growth abnormalities and an increased risk of childhood tumors. Several types of childhood tumors including Wilms’ tumor, adrenocortical carcinoma and rhabdomyosarcoma display a specific loss of maternal 11p15 alleles, suggesting that genomic imprinting plays an important part. This region also contains two other imprinted genes, insulin-like growth factor II (IGF-II) and H19, both of which seem to be implicated in adrenal neoplasms. Applications: Suitable for use in Immunohistochemistry. Not suitable for use in Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Formalin/paraffin): 1:400-1:800 for 20 minutes at RT. Immunohistochemistry (Formalin/paraffin): 1:200 using Ultravision Quanto Detection System for 20 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Colon carcinoma or placenta Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice