K1849-01C

Kip1, p27 (cdk Inhibitor, Cyclin Dependent Kinase Inhibitor)

Size

£812.00

SKU:: K1849-01C
Application:: IHC-Fr
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to Met1-Thr198 from human p27/Kip1, expressed in E. coli.
Clone:: 9H146
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27 Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 8-25ug/ml detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.