K1800-05
Kininogen (KNG) (Not for Export EU)
Size
£607.00
- SKU:
- K1800-05
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Purified kininogen from pooled human plasma
- Format:
- Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further with PBS, pH 7.2.
- Extra Details:
- Human plasma contains at least two distinct forms of kininogen, the carrier molecule of bradykinin, which can be distinguished by their physical, chemical and immunochemical properties. They are designated high-molecular-weight kininogen (HMWK) and low-molecular weight kininogen (LMWK). HMWK (MW 110000) and LMWK (MW 50000) both liberate bradykinin following the action of kallikrein. HMWK circulates as a non-enzymatic factor that is central to contact activation reactions. LMWK plays no known role in the activation of coagulation. Normal adult plasma levels are 7mg/100ml for HMWK as determined by electroimmunodiffusion and 9mg/100ml based on haemagglutination inhibition assays and RIA. Low plasma kininogen levels are seen in newborn and pre-term infants. HMWK is absent in congenital kininogen deficiency of the named affected families Fitzgerald and Reid; Williams and Flaujeac lack both HMWK and LMWK. The condition is associated with the decreased fibrinolytic activity and kinin formation but is asymptomatic. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice