K1797-50

Kinetoplastid Membrane Protein 11 (KMP-11, Lipophosphoglycan-associated Protein, LPGAP)

Size

£1,240.00

SKU:: K1797-50
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: L. donovani lipophosphoglycan semi purified from epimastigotes.
Clone:: L157
Format:: Supplied as a lyophilized powder. No preservative added. Reconstitute with 500ul sterile ddH2O.
Extra Details:: Kinetoplastid Membrane Protein-11 (KMP-11), previously known as the lipophosphoglycan-associated protein (LPGAP), is a 92 amino acid, ~11kD membrane protein. K1797-50 was originally derived against Leishmania donovani lipophosphoglycan, but found to recognize the tightly associated LPGAP. KMP-11 is found in a wide variety of Kinetoplastid parasites including African trypanosomes, Leishmania and Crithidia. Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilution: ELISA: 1:2000 Western Blot: 1:1000-1:3000 Immunofluorescence: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.