K1700-11A

Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation Related Ki-67 Antigen)

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£691.00

SKU:: K1700-11A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to an internal portion (within residues 1600-1700) of mouse Ki-67 protein (Q61769). Cellular Localization: Nuclear
Format:: Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.09% sodium azide.
Extra Details:: Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki67 index and histologically low-grade tumors is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:10-1:500 Western Blot: 1:100-1:2000 Immunohistochemistry (Paraffin): 1:100-1:500 Immunohistochemistry (Frozen): 1:100-1:500 Flow Cytometry: 1:100 Optimal dilution determined by the researcher. Positive Controls: Mouse intestine, pancreas, spleen Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice