K1000-07-FITC
Keratinocyte Growth Factor (KGF, Fibroblast Growth Factor 7, FGF-7) (FITC)
Size
£843.00
- SKU:
- K1000-07-FITC
- Purity:
- Purified by Immunoaffinity chromatography. Endotoxin: <0.1ng/ug protein
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human Keratinocyte Growth Factor expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- Applications: Suitable for use in FLISA, Neutralization, and Western Blot. Other applications not tested. Recommended Dilution: FLISA (Indirect): 0.5-2ug/ml. Using 100ul/well antibody solution. When used in conjunction with the compatible secondary reagents, the detection limit is of 0.2-0.4ng/well. FLISA (Sandwich): 0.5-2ug/ml. Using 100ul/well antibody solution. When used in conjunction with K1000A, as a detection antibody, the detection limit is of 0.2-0.4ng/well. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: 0.5ug/ml, overnight at 4°C. This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. An HRP-labeled polymerdetection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Neutralization: 0.56-0.85ug/ml, to yeild one-half maximal inhibition [ND50] of the biological activity of hKGF (25ng/ml). Optimal dilution determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice