Keyhole Limpet Hemocyanin (KLH) (HRP)

Size

£403.00

SKU:: K0300-08
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: Purified Keyhole Limpet Hemocynanin (KLH).
Format:: Supplied as a liquid in PBS, pH 7.2, 0.01% thimerosal, 20% glycerol. Labeled with Horseradish peroxidase (HRP).
Extra Details:: Keyhole Limpet Hemocyanin (KLH) is generally a very efficient coupling agent. It is the most common carrier protein used in the preparation of hapten conjugates. KLH is composed of five subunits. It is lysine rich with a large number of available primary amines to facilitate conjugation, antibody production and promote peptide attachment after dissociation. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1903-80: IgY, H&L, X-Adsorbed, 12nm (Gold) Pab Dk x Ch I1903-80A: IgY, H&L, X-Adsorbed, 18nm (Gold) Pab Dk x Ch I1903-80B: IgY, H&L, X-Adsorbed, 6nm (Gold) Pab Dk x Ch I1906: IgY Pab Rb x Ch I1906-01: IgY, Fc (HRP) Pab Gt x Ch I1906-10: IgY (HRP) Pab Rb x Ch I1906-12: IgY (FITC) Pab Rb x Ch I1906-14: IgY (AP) Pab Rb x Ch Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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