Keyhole Limpet Hemocyanin (KLH) (FITC)

Size

£856.00

SKU:: K0300-07
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: KLH (Keyhole Limpet Hemocyanin)
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 100ul sterile ddH2O.
Extra Details:: Applications: Suitable for use in FLISA, Immunomicroscopy, Flow Cytometry, FACS, Immunofluorescence and Fluorescent Western Blot. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:500-1:2500 Optimal dilutions to be determined by the researcher. Fluorochrome/Protein Ratio: 3.4 moles FITC per mole of Rabbit IgG Excitation Wavelength: 495nm Emission Wavelength: 528nm Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.