K0199-91

Keratin, pan multiple clones

Size

£576.00

SKU:: K0199-91
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Clone:: PAN-CK Cocktail
Format:: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Extra Details:: Keratins are resolved with two dimensional gel electrophoresis into acidic pH, <5.7, and basic, pH >6.0 subfamilies. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Applications: Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Frozen & formalin/paraffin): 1-2ug/ml. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT, or Pronase E at 1mg/ml PBS for 5 minutes at 37°C. Optimal dilutions to be determined by the researcher. Positive Control: Skin Cellular Localization: Cytoplasmic Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.