K0199-51
Keratin 5
Size
£499.00
- SKU:
- K0199-51
- Application:
- IHC-Fr
- Purity:
- Culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to C-terminal 103aa of human Keratin 5.
- Clone:
- XM26
- Format:
- Supplied as a liquid in 0.09% sodium azide.
- Extra Details:
- Twenty human keratins are divided into acidic (pI <5.7) and basic (pI >6.0) subfamilies. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor identification. Point mutations in cytokeratin5 gene can cause various types of epidermolysis bullosa simplex. It is expressed in most epithelial and biphasic mesotheliomas. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin/paraffin): 1:20 for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilution determined by the researcher. Positive Control: Mesothelioma Cellular Localization: Cytoplasmic Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice