K0199-25
Keratin 20
Size
£499.00
- SKU:
- K0199-25
- Purity:
- Tissue culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Semi-purified human cytokeratin. Molecular Weight of Antigen: 46kD. Cellular Localization: Cytoplasmic.
- Clone:
- 0.N.367
- Format:
- Supplied as a liquid in 0.09% sodium azide.
- Extra Details:
- CK20 is a Type-I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. CK20 is expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system. CK20 is also present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack in CK20. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml for 2hrs at RT Immunohistochemistry (formalin/ paraffin): 1:50-1:100 for 30 min at RT. Requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C. Optimal dilutions to be determined by the researcher. Positive Control: HT29 cells. Colon carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice