K0199-24E

Keratin 19 (KRT19, CK19, CK-19, Cytokeratin-19, K19, K1CS, Keratin, type I cytoskeletal 19, Keratin-19, MGC15366)

Size

£512.00

SKU:: K0199-24E
Purity:: Purified by Protein G affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Detergent soluble extract of human mammary epithelium.
Clone:: BA17
Format:: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Extra Details:: Cytokeratin 19 is a member of type I acidic subfamily of intermediate filaments. It is expressed in various different human tissues except in liver. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2-4ug/ml for 2 hrs at RT Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilution determined by the researcher. Positive Control: Ls174T and HT29 Cells, Colon ca. Cellular Localization: Cytoplasmic Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.