K0199-18
Keratin 15 (KRT15, CK15, CK-15, Cytokeratin-15, K15, K1CO, Keratin, type I cytoskeletal 15, KRTB)
Size
£716.00
- SKU:
- K0199-18
- Application:
- IF
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to 17 residues from the C-terminal of human cytokeratin 15. Cellular Localization: Cytoplasmic.
- Clone:
- 0.N.360
- Format:
- Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
- Extra Details:
- Keratin 15 is a type I keratin without a defined type II partner. Keratin 15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Expression of keratin 15 is downregulated in some hyperproliferating situations, such as psoriasis and hypertrophic scars. Because keratinocytes in psoriasis and hypertrophic scars are activated, it is suggested that keratin 15 expression is not compatible with keratinocyte activation and the keratin 15 gene is downregulated to maintain the activated phenotype. Applications: Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (FFPE): 1:15 for 20 minutes at RT. Boiling tissue sections in 1mM EDTA, pH 8.0 for 10-20 minutes followed by cooling at RT for 20. minutes is required. Optimal dilutions to be determined by the researcher. Positive Control: Skin Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice