K0199-17

Keratin 14

Size

£544.00

SKU:: K0199-17
Purity:: Purified by Protein G affinity chromatography from ascites
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to 15aa from the C-terminus of human keratin 14. Cellular Localization: Cytoplasmic
Clone:: 0.N.359 (LL002)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Extra Details:: Keratin 14 belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5. Keratin 14 has been studied as a prognostic marker in breast cancer. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml for 2 hours at RT. Detects a band at ~50kD. Immunohistochemistry (Formalin/paraffin): 1:800-1:1600 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: A431 cells; Skin or Squamous cell carcinoma Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.