K0199-10
Keratin 8
Size
£895.00
- SKU:
- K0199-10
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Keratin preparation from a human carcinoma.
- Clone:
- 0.N.351
- Format:
- Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide.
- Extra Details:
- Applications: Suitable for use in Immunohistochemistry (formalin/paraffin) and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Immunoprecipitation: 2ug antibody/mg protein lysate. Use Protein G. Immunohistochemistry (formalin-fixed, paraffin embedded tissues): 0.5-1ug/ml for 30 mins. at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice