K0199-05T

Keratin 5, 18 (Keratin Type II Cytoskeletal 5, Keratin 5, Keratin-5, K5, KRT5, KRT5A, Keratin Type I Cytoskeletal 18, Keratin 18, Keratin-18, K18, KRT18, 58kD Cytokeratin, Cell Proliferation-inducing Gene 46 Protein, Cytokeratin-5, CK5, CK-5, Cytokeratin-18, CK-18, CYK18, DDD, EBS2, PIG46, Type-II Keratin Kb5)

Size

£601.00

SKU:: K0199-05T
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Cytoskeleton preparation from HeLa cells.
Clone:: C-50
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Applications: Suitable for use in Immunoprecipitation, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 10ug/ml. Proteolytic digestion of the tissue is essential. Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human prostate. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.