KAP-1 (TRIM28, KAP1, TF1B, RNF96, TIF1B, tripartite motif-containing 28, nuclear corepressor)

Size

£642.00

SKU:: K0075-05A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to 13aa, C-SSQELSGGPGDGP, from near the C-terminus of human TRIM28.
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:8000. Western Blot: Approx 100-120kD band seen in HepG2 lysate. Recommended for use at 0.2-1ug/ml (Predicted MW approx 92kD according to NP_005753). Immunohistochemistry: 0.1ug/ml. Successfully used in IHC assays on human gastric cancer surgical specimens: Yokoe et al, Ann Surg Oncol. 2009 Nov 7. [Epub ahead of print]. (PMID: 19898899.) Immunofluorescence: 5ug/ml. Successfully used in on mouse embryo at the late zygote stage Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.