K0005-16
Kallikrein 13 (KLK13)
Size
£837.00
- SKU:
- K0005-16
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- NS0-derived rhKLK13 (aa19-262)
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Applications: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human KLK13. The detection limit for rhKLK13 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rhKLK13 from conditioned media of transfected NS0 cells. The recovered rhKLK13 can be detected by western blot analysis using a monoclonal antibody. Neutralization: This antibody was used to inhibit/neutralize the enzymatic activity of rhKLK13. The antibody was preincubated with the enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate, Boc-VPR-AMC. 50% of the proteolytic activity was inhibited by the antibody at approximately 8.6ug/ml (IC50) under conditions in which the enzyme was present at 1ug/ml and the substrate concentration was 100 μM. Considering the molecular masses of the enzyme (28 kD) and the antibody (150 kD), IC50 was achieved at approximately 2:1 molar ratio of the antibody to the enzyme. Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human KLK13. The detection limit for rhKLK13 is approximately 1.0 ng/well. Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice