K0005-14B

Kallikrein 11 (KLK11, Hippostasin, Trypsin-like serine protease and PRSS20)

Size

£863.00

SKU:: K0005-14B
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: NS0-derived recombinant protein corresponding to Ile54-Asn282 from human Kallikrein 11.
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Kallikrein 11 is expressed in brain, skin, ovary and prostate. Increased levels of Kallikrein 11 have been reported in prostate and ovarian carcinoma. It is a serine protease. Kallikrein 11 (KLK11), also known as hippostasin, trypsin-like serine protease and PRSS20, is a member of human tissue kallikrein family.6 Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences, respectively.1-4. Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain (residues 22-250). Isoform 2 is identical to isoform 1, except that a 32 amino acid segment is inserted in isoform 2 before residue1 in isoform 1. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas.7-9 rhKLK11, after being activated by thermolysin, is active against a thioester substrate. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western blot: 0.1ug/ml Immunohistochemistry: 5-15ug/ml in immersion fixed paraffin-embedded sections of human prostate. Immunoprecipitation: 25ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice