K0004
Kaiso (Zinc Finger and BTB Domain Containing 33, ZBTB33)
Size
£800.00
- SKU:
- K0004
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant N-terminal GST and His-tagged fusion protein corresponding to aa103–584 at the central region of mouse Kaiso.
- Clone:
- 2T15 (6F)
- Format:
- Supplied as a liquid in Tris-glycine, pH 7.4, 0.05% sodium azide before the addition of glycerol to 30%.
- Extra Details:
- Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Supershift. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: 1ug/ml gives positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100% methanol. Western Blot: 0.5-2ug/ml detects Kaiso in RIPA lysates from 3T3/A31 cells. Immunoprecipitation: 2-5ug immunoprecipitates Kaiso from RIPA lysates from NIH 3T3 cells. Supershift: Supershifts a specific methyl-CpG complex. Optimal dilutions to be determined by the researcher. Positive Antigen Control: F4209: 3T3/A31 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice