J8025

Junctional Adhesion Molecule C (JAM-C)

Size

£819.00

SKU:: J8025
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to soluble mouse JAM-C
Clone:: 6A590 (CRAM-19H36)
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Extra Details:: Junctional adhesion molecule-C (JAM-C) also known as JAM-2 is a 45kD cell adhesion molecule CAM). JAM-C is a transmembrane protein which is a member of the immunoglobulin superfamily found at intercellular junctions of endothelial cells. JAM-C belongs together with JAM-A (JAM or JAM-1) and JAM-B (VE-JAM or JAM-3) to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-C is potentially involved in the junctional sealing of the vascular endothelium, in particular of high endothelial venules (HEV). In adult murine tissue JAM-C expression is reported to be restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. In humans, JAM-C expression is not restricted to endothelial cells, but is also expressed on human lymphocytes. Applications: Suitable for use in ELISA, Immunoprecipitation, Flow Cytometry and Immunohistochemistry. Not suitable for Western Blot or Immunohistochemistry on paraffin sections. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1:50 Immunohistochemistry (frozen): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice