J8025-01

Junctional Adhesion Molecule C (JAM-C, JAM-2)

Size

£819.00

SKU:: J8025-01
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to mouse soluble JAM-C.
Clone:: CRAM-18 F26
Format:: Supplied as a liquid in PBS, containing 0.1% BSA.
Extra Details:: Junctional adhesion molecule-C (JAM-C) also known as JAM-2 is a 45kD cell adhesion molecule (CAM). JAM-C is a transmembrane protein which is a member of the immunoglobulin superfamily found at intercellular junctions of endothelial cells. JAM-C belongs together with JAM-A (JAM or JAM-1) and JAM-B (VE-JAM or JAM-3) to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-C is potentially involved in the junctional sealing of the vascular endothelium, in particular of high endothelial venules (HEV). In adult murine tissue JAM-C expression is reported to be restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. In humans, JAM-C expression is not restricted to endothelial cells, but is also expressed on human lymphocytes. Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry (frozen) and Immunoprecipitation. Also useful for inhibition of biological activity. Not suitable for use in WB and Immuohistochemistry on paraffin sections. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen): 1:50 Flow Cytometry: 1:50 For inhibition of biological activity dilutions have to be made according to the amounts of JAM-C to be inhibited. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice