Junctional Adhesion Molecule 1 (JAM-1, CD321, F11 Receptor, F11R, Junctional Adhesion Molecule A, JAMA, JCAM, KAT, Platelet Adhesion Molecule 1, PAM1, Platelet F11 Receptor, UNQ264/PRO301) (MaxLight 650)

Size

£953.00

SKU:: J8009-ML650
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the C-terminal of human JAM-1 protein
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
Extra Details:: MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. The junctional adhesion molecule-1 (JAM-1) is an integral membrane protein that colocalizes with tight junction molecules occludin, ZO-1, and cingulin.1 JAM-1 has been characterized as a member of the immunoglobulin (Ig) superfamily and is composed of an extracellular domain, a single-pass transmembrane region, and a short cytoplasmic tail.2 JAM-1 is localized at tight junctions in endothelial and epithelial cells and its sub-cellular distribution is modulated by inflammatory cytokines.2 JAM-1 is also expressed on circulating leukocytes and platelets as well as in lymphoid.2 Most claudins as well as JAM-1 end with the amino acid Val at their C-terminal ends, suggesting that these COOH termini directly bind to PDZ domains.3 JAM-1 appears to be a single copy gene, which is constitutively expressed on various tissues and cells, and may be involved in cell-to-cell adhesion through homophilic interaction.4 JAM-1 was detected in human intestine cell lines T84, CaCO2 and HT29, in the lung cell line A 549, and the liver cell line HepG2.5 Applications: Suitable for use in Western Blotting. Other applications not tested. Recommended Dilution: Western Blotting: 1–3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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