Junctional Adhesion Molecule 4 (JAM4, Immunoglobulin Superfamily Member 5, IGSF5, Cortical Thymocyte Marker in Xenopus, CTX) (Biotin)

Size

£908.00

SKU:: J8009-30
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: NS0-derived recombinant protein corresponding to Leu15-Ala139 from mouse JAM‑4/IGSF5.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
Extra Details:: JAM-4, also known as IGSF5, is a 93kD member of the CTX (Cortical Thymocyte Marker in Xenopus) subfamily of the Ig superfamily. It is a type I transmembrane protein with an N-terminal extracellular V-type and C2 type Ig domain separated by a J segment, a transmembrane domain and a cytoplasmic tail with a PDZ binding motif. JAM-4 is expressed in intestinal, renal, and mammary epithelial cells, spermatogonia, and hematopoietic stem cells. It is a cell adhesion molecule that mediates homophilic interaction. JAM-4 also interacts with the intracellular membrane-associated guanylate kinase inverted 1 (MAGI-1). An additional isoform containing a 100 residue insertion between Gln 125 and Asp 126 has been reported. Over the region used as immunogen, mouse JAM-4 shares 82% and 63% amino acid sequence identity with rat and human JAM-4, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml with recombinant mouse JAM‑4/IGSF5 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice