J8009-02A

Junctional Adhesion Molecule 1 (Junctional Adhesion Molecule A, JAM-A, JAM-1, Platelet Adhesion Molecule 1, PAM-1, CD321, Platelet F11 Receptor, F11R, JAM1, JCAM, F11R, UNQ264/PRO301) (APC)

Size

£787.00

SKU:: J8009-02A
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Ser28-Ala242 (predicted) from human JAM-A expressed in NSO cell line.
Clone:: 12K120
Format:: .Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Extra Details:: The members of junctional adhesion molecules family are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily. Human JAM-A, also known as platelet adhesion molecule 1 (PAM-1) and platelet F11 receptor, is predominantly expressed at intercellular junctions of both epithelial cells and endothelial cells. It is also expressed on circulating blood cells including neutrophils, monocytes, platelets, erythrocytes, and lymphocytes. JAM-A exhibits homophilic interactions to regulate tight junction assembly and modulate paracellular permeability. This homophilic interaction also mediates platelet aggregation and adhesion to endothelial cells and may play a role in thrombosis. The human and mouse proteins share ~67% aa sequence homology. Human JAM-A also shares ~35% and 32% aa sequence homology with human JAM-B and JAM-C. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells in MCF-7 human breast cancer cell line Optimal dilutions to be determined by the researcher. Excitation: 620-650nm Emission: 660-670nm Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice