J8000-09

JunD (Jun D proto-oncogene, AP-1, Transcription factor jun-D)

Size

£825.00

SKU:: J8000-09
Application:: WB
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant human JunD, aa47-156; P17535 (E. coli).
Clone:: 9H99
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: JunD is a member of the Jun family of nuclear transcription factors. It dimerizes with other Jun or Fos family members to form the AP1 transcription factor. Unlike c-Jun or JunB, JunD antagonizes Ras transformation and accumulates as cells enter quiescence. JunD is a 347aa, 39kD protein (JunD-FL) that has a 303aa, 34kD isoform (delta JunD) which begins at aa44. Over the sequence used as an immunogen, human JunD shares 72% aa identity with mouse and rat JunD. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.