Jumonji/ARID Domain Containing Protein 2 (JARID2, JARD2, JMJ, Jumonji AT Rich Interactive Domain 2, Jumonji Protein)

Size

£594.00

SKU:: J7876-20
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa1-100 of human JARID2, at N-terminal.
Format:: Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: JARID2 or the Jumonji protein is a PRC2 component in embryonic stem cells required for multi-lineage differentiation and recruitment of PRC1 and RNA Polymerase II to developmental regulators. It is essential for embryogenesis, including neural tube formation. It plays a key role in differentiation of embryonic stem cells and normal development. Defects in JARID2, characterized by PRC1 and PRC2 mutants, show a severely compromised capacity for successful differentiation towards neural or mesodermal fates. JARID2 is an excellent marker for studying differentiation patterns in induced pluripotent stem cells (iPSCs) and embyonic stem cells (eSCs). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence (IC): 1:50-1:200 Western Blot: 2ug/ml Immunocytochemistry: 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice