Jo-1 (Histidyl t-RNA Synthetase)

Size

£512.00

SKU:: J5000
Purity:: Purified by fractionation of delipidated and defibrinated donor human serum.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Human
Immunogen:: N/A. This product is Jo-1 Autoantibody derived from donor human plasma.
Format:: Supplied as a lyophilized powder from 0.01M PBS, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.
Extra Details:: Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA). This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA. The most prominent of ANAs/ENAs are autoantibodies which binds to ds-DNA, ss-DNA, histones, ribonucleoproteins (RNP) and the SS-A, SS-B, Sm antigens, Jo-1, and Scl-70. Two antibodies, anti-dsDNA and anti-Sm, appear to occur only in SLE. Others occur in a variety of autoimmune and mixed connective tissue diseases. A heterogeneous group of precipitating antibodies called anti-Jo-1 have been found in dermatopolymyuositis. Anti-Jo is present in approx. 25% of patients with myositis. Recently, Jo-1 has been demonstrated to be an RNA-associated 50kD polypeptide antigen. It has been identified as histidyl-tRNA-synthetase, the RNA being tRNA-his. The frequency of ANA-positives in various rheumatic diseases has been reported for SLE, rheumatoid arthritis (RA), progressive systemic sclerosis (PSS), polymyositis (PM), dermatomyositis (DM), mixed connective tissue diseases, drug-induced SLE, and Sjogren’s syndrome (SS). Most of these studies are based on tedious fluorescent ANA (FANA). Other techniques such as RIA, immunodiffusion, hemagglutination, electrophoresis and immunoblotting are also used to define antibody specificity. Recently, immunological assays (mostly ELISA) that determine the specificity of ANA have been used in studying patients with systemic rheumatic diseases. Applications: Suitable for use in ELISA, Immunodiffusion and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA: 1:100 Optimal dilutions to be determined by the researcher Titer: Immunodiffusion: 1:4 Autoantibody Activity: Activity by ELISA: Current lot value: 1577EU/ml Interpretation of Results: <16 EU/ml: Negative; 16-20EU/ml: Equivocal; Positive; >20 EU/ml: SSA: 1.6EU/ml SSB: 0.7EU/ml SCL: 4.6EU/ml SM: 0.7EU/ml Sm/RNP: 1.9EU/ml Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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