J4998-10A

Jo-1 (Histidyl t-RNA Synthetase)

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£1,643.00

SKU:: J4998-10A
Purity:: Immunoaffinity purified from calf and/or rabbit thymus through the use of immobilized human anti-Jo-1 immune globulins. Other antigens (Sm, RNP, SSA, Scl-70, and SSB) are not detected with ELISA assays. Homogeneity is verified by SDS PAGE.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 0.01M Tris, 0.53M sodium chloride, 0.02% sodium azide, 50% glycerol.
Extra Details:: The Jo-1 antigen is histidyl-transfer ribonucleic acid (t-RNA) synthetase. This enzyme is partially responsible for attaching t-RNA to their cognate rRNA (2). The Jo-1 antigen migrates as a 53 kD protein on SDS-PAGE. Anti-Jo-1 autoantibodies were originally described as precipitating autoantibodies in sera of patients with polymyositis. It was later realized that the anti-Jo-1 antibodies were specific for patients with polymyositis. The target for the anti-Jo-1 antibodies was one of a family of distinct cellular enzymes, the aminoacyl t-RNA synthetases (1). The presence of autoantibodies against the Jo-1 antigen has been reported in up to 23% of polymyositis patients by immunodiffusion (3). Anti-Jo-1 antibodies are almost completely specific for myositis, being more common in polymyositis than dermato-myositis(4), and rare in children (5). The presence of anti-Jo-1 antibodies defines a distinct group of polymyositis patients with interstitial disease, arthritis, and fevers (6). The anti-Jo-1 response appears to be self-antigen driven, having a broad spectrotype over time undergoing isotype switching. Anti-Jo-1 antibodies also inhibit the function of human histidyl tRNA synthetase more than they do from other species (6). Source: Rabbit thymus Unit Definition: One unit of activity is the amount of antigen that will generate an absorbance of 1.2 when assayed using standard procedure for enzyme immunoassay. Activity will vary with assay conditions/protocols. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Dry Ice