J2475-01A-FITC

JNK1 (c-Jun N-terminal Kinase 1, JNK1A2, JNK, JNK21B1/2, JNK-46, Mitogen-activated Protein Kinase 8, MAP Kinase 8, MAPK 8, MAPK8, PRKM8, Stress-activated Protein Kinase 1c, SAPK1c, SAPK1, Stress-activated Protein Kinase JNK1) (FITC)

Size

£914.00

SKU:: J2475-01A-FITC
Purity:: Purified by Protein A chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant GST-fusion protein of JNK1
Clone:: 4A2G12
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: The mitogen-activated protein kinase (MAP) kinase family includes ERK, JNK and p38 kinase. The pathway, regulated by (MAP) kinase family, involves a broad array of cellular responses that include survival, cell proliferation, and apoptosis. The JNK signal transduction pathway is activated in response to environmental stress and by engagement of several classes of cell surface receptors including cytokine receptors, serpentine receptors and receptor tyrosine kinases. Although JNK is located in both the cytoplasm and the nucleus of quiescent cells, activation of JNK is associated with accumulation of JNK in the nucleus. In the NGF (nerve growth factor)-differentiated PC-12 cells, activation of the ERK signaling pathway promotes cell survival but stimulation of the JNK and p38 signaling pathways contributes to cell death. Applications: Suitable for use in Immunofluorescence and Western Blot. Other applications have not been tested. Recommended Dilution: Immunofluorescence: 5-10ug/ml Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice