J Chain (Not for Export EU)

Size

£594.00

SKU:: J1255-01
Purity:: Serum.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: J chain released and purified from dimeric human myeloma IgA by sulphitolysis.
Format:: Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O.
Extra Details:: Human J chain is a polypeptide folded within the polymeric structure of the immunoglobulin. J chain isolated from human polymeric IgA and IgM are identical by criteria of composition, peptide maps and antigenicity. Human J chain has been established as distinct from all other component chains of polymeric IgA and IgM. It has a unique primary structure. Antisera to light chains or heavy chains of human Immunoglobulins do not react with J chain. J chain is released from dimeric human myeloma IgA by sulphitolysis and purified by ion exchange chromatography in urea and gel filtration in guanidine. Its purity is tested in alkaline urea polyacrylamide gel electrophoresis, in immunodiffusion and immunoelectrophoresis, showing it to be free of IgA, heavy or light chain contaminants. Applications: Suitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion. Other applications not tested. Recommended Dilutions: Double Radial Immunodiffusion: Use a rosette arrangement with 10ul in 3mm diameter center well and 2ul serum samples (neat and serially diluted in 2mm diameter peripheral wells). Note: J1255-01 should be applied first, followed by free J chain or Reference Solution. Normal human serum can be used as a negative control. Immunoelectrophoresis: 120ul against 2ul serum or equivalent Optimal dilutions to be determined by the researcher. Supplied with: J1255-01B: Reference Solution, 1x100ul. Reconstitute with 100ul dH2O. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice