JAW1 (Lymphoid-restricted Membrane Protein, Protein Jaw1, LRMP)

Size

£876.00

SKU:: J1050-01A
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Gln143-Pro292 from human LRMP expressed in E.coli.
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: LRMP is a 74-76kD protein that is associated with the ER. In contrast to its name, LRMP expression is not restricted to lymphoid cell types. Cells known to express LRMP include stomach chief cells, seminal vesicle cuboidal epithelium, neurons, germinal center B cells, cortical thymocytes, and cells of the taste buds. LRMP is suggested to participate in intracellular peptide transport. It is also reported to physically interact with IP3RP. Human LRMP is a 56kD, single-pass transmembrane (TM) protein that is 499aa in length. It contains a 439aa cytoplasmic region aa1-439, a 21aa TM segment aa440-460 and a 39aa luminal domain aa461-499 that is cleaved after Ala469 to generate a type IV TM protein. LRMP runs anomalously in SDS-Page, and the 75kD form may be linked to an isoform that utilizes a Leu start site 56aa upstream of the start site for the 499aa isoform. In this regard, a human isoform has been reported that is 62kD in size. Mature mouse LRMP is 509aa in length (after cleavage) and runs at 65kD in SDS-PAGE. Over aa143-292, human LRMP shares 93% aa identity with mouse LRMP. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml with lysates of Ramos human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice