J0902-16

Jak3, N-terminal GST-Tag, Recombinant, Human (Janus Kinase 3, EC 2.7.10.2, JAKL, Leukocyte Janus Kinase, LJAK)

Size

£907.00

SKU:: J0902-16
Molecular Weight:: 65
Purity:: Purified ≥60%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 25mM Tris-HCl, pH8.0, 100mM NaCl, 0.05% tween-20, 1mM DTT, 20% glycerol.
Extra Details:: Source: Recombinant corresponding to aa781-end of human Jak3 with N-terminal GTS tag expressed in Baculovirus expression sstem (NM_000215). Specific Activity: 118pmol/min/ug. Assay conditions: 50mM HEPES, pH7.5, 10mM MgCl2, 1mM EGTA, 200uM ATP, 0.01% Brij-35, 2uM substrate at RT for 1hour. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.