J0902-04A
JAK3 (Janus Kinase 3, EC 2.7.10.2, JAKL, Leukocyte Janus Kinase, LJAK)
Size
£825.00
- SKU:
- J0902-04A
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gly46-Thr209 from human Jak3, expressed in E. coli.
- Clone:
- 9H144
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Janus Kinase 3 (Jak3) belongs to the Jak family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Also known as Leukocyte Janus Kinase (LJak), Jak3 is activated after binding to the gamma chain of Interleukin receptors, specifically the IL-2 and IL-4 receptors. Activation of Jak3 is associated with the rapid tyrosine phosphorylation of STAT proteins. Defects in Jak3 cause an autosomal severe combined immunodeficiency disease (SCID). Applications: Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug/10e6 cells Immunocytochemistry: 8-25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice