J0900-03
JAK1 (Janus Kinase 1) (Not for Export EU)
Size
£767.00
- SKU:
- J0900-03
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic HPLC-purified (≥98%) peptide corresponding to 21aa (KTLIEKERFYESRCRPVTPS-C) of human JAK1.
- Format:
- Supplied as a liquid, 0.02% sodium azide.
- Extra Details:
- Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs. Applications: Suitable for use in Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Immunoprecipitation: 5ul per 1-2mg of RIPA cell lysate. Tested using a RIPA lysate from mouse CTLL lymphocytes, TF-1 erythroleukemic cells and mouse thymus. Note: The amount of JAK1 in cell lines tends to be extremely low. It is highly recommended that a RIPA cell lysate (1-2mg protein) be prepared. JAK1 should be first concentrated by Immunoprecipitation (5ul JAK1 antiserum) and then Western Blot (1:500 dilution). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice