ISL1 (IISL LIM Homeobox 1, Insulin Gene Enhancer Protein ISL-1, Isl-1, ISLET1, Islet-1)

Size

£819.00

SKU:: I8782-60A
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated linear peptide corresponding to human ISL-1. Epitope: Internal region of ISL-1. Species Sequence Homology: equine, bovine, sheep, rat and canine; 100%.
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide
Extra Details:: Islet-1 (ISL-1) is a transcription factor that plays an important role in the embryogenesis of pancreatic islets of Langerhans. It is expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain. In mouse embryos, a deficiency of this gene fail to undergo neural tube motor neuron differentiation. More recently it has been identified as a marker for a cardiac progenitor cell lineage that is capable of differentiating into all 3 major heart cell types: cardiomyocytes, smooth muscle and endothelial cell lineages. Applications: Suitable for use in Immunocytochemistry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml detected ISL-1 on 10ug K562 cell lysate. Immunohistochemistry (Paraffin): 1:300 detected ISL-1 in mouse pancreas tissue. Antigen retrieval with citrate, pH 6.0 is recommeneded. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice