I8700-05Q
IRS1 (Insulin receptor substrate 1, HIRS-1, Insulin receptor substrate 1, IRS-1)
Size
£800.00
- SKU:
- I8700-05Q
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ala801-Ala1020 (predicted) from human IRS1 expressed in E. coli.
- Clone:
- 11n114
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Human IRS1 (insulin receptor substrate 1) is a 160-180kD substrate intermediate between the insulin and IGF-I receptor, and downstream signaling modulators. Upon insulin/IGF-I receptor activation, IRS1 is tyrosine phosphorylated, allowing its association with PI-3 kinase and GRB2. Human IRS1 is 1242aa in length. It contains a PH (pleckstrin homology) domain (aa12-115), followed by a PTB (phosphotyrosine-binding) domain (aa160-263) and ten PEST (Pro/Glu/Ser/Thr) regions (aa340-1225). IRS1 may be proteolytically cleaved at aa656-657, generating a 90kD and 79kD fragment. Over aa801-1020, human IRS1 is 88, 86-88aa identical to dog, mouse and pig IRS1, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice