I8700-01D

IRS-1 (Insulin Receptor Substrate 1)

Size

£812.00

SKU:: I8700-01D
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: E. coli-derived recombinant protein corresponding to Ala801-Ala1020 (predicted) from human IRS-1.
Clone:: 7H204
Format:: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Insulin Receptor Substrate 1 (IRS-1) is a cytoplasmic protein that participates in the insulin signaling pathway. It is phosphorylated by the Insulin receptor upon binding of Insulin and subsequently associates with several SH2-domain-containing proteins. Applications: Suitable for use in Immunocytochemistry, Western Blot and ELISA. Other applications not tested. Recommended Dilution: Immunocytochemistry: 10ug/ml Western Blot: 1-2ug/ml ELISA: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.