I8604-18F

IRE1 (Serine/Threonine-protein Kinase/Endoribonuclease IRE1, Endoplasmic Reticulum-to-nucleus Signaling 1, Inositol-requiring Protein 1, hIRE1p, Ire1-alpha, IRE1a, ERN1)

Size

£642.00

SKU:: I8604-18F
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-PNAHGKIKAMISD, from human IRE1, at the internal region of the protein. Species sequence homology: Bovine, canine, mouse, porcine and rat.
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. Applications: Suitable for use in ELISA, Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:16,000 Western Blot: 0.1-0.3ug/ml, observed in human pancreas and duodenum lysates, ~110kD band Flow Cytometry: 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.