I8600-18B-ML490

IRAK 4, CT ( (IL-1R-associated Kinase 4, NY-REN-64, REN64) (MaxLight 490)

Size

£902.00

SKU:: I8600-18B-ML490
Purity:: Purified by immunoaffinity chromatography from whole serum.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A peptide corresponding to amino acids at the carboxy terminus of human IRAK4.
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Extra Details:: MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. IRAK4 has a molecular weight of 50kD, and is an important mediator in the signal transduction of Toll-like receptor (TLR) and interleukin-1 (IL-1) receptor family members. IRAK4 functions in this signal transduction pathway by binding to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signalling cascades leading to transcriptional up-regulation and mRNA stabilization. IRAK4 is a key activator of TLR-mediated signal transductions and has crucial roles in innate immune responses. It is expressed by the liver, kidneys and skeletal muscle. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml ~50kD in HeLa cell lysates. Immunohistochemistry Frozen: 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice