I8600-18B-HRP
IRAK 4, CT ( (IL-1R-associated Kinase 4, NY-REN-64, REN64) (HRP)
Size
£902.00
- SKU:
- I8600-18B-HRP
- Purity:
- Purified by immunoaffinity chromatography from whole serum.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A peptide corresponding to amino acids at the carboxy terminus of human IRAK4.
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- IRAK4 has a molecular weight of 50kD, and is an important mediator in the signal transduction of Toll-like receptor (TLR) and interleukin-1 (IL-1) receptor family members. IRAK4 functions in this signal transduction pathway by binding to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signalling cascades leading to transcriptional up-regulation and mRNA stabilization. IRAK4 is a key activator of TLR-mediated signal transductions and has crucial roles in innate immune responses. It is expressed by the liver, kidneys and skeletal muscle. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml ~50kD in HeLa cell lysates. Immunohistochemistry Frozen: 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice