I8600-17C-ML490

IRAK 4 (IL-1 Receptor Associate Kinase 4) (MaxLight 490)

Size

£1,106.00

SKU:: I8600-17C-ML490
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide derived from the N-terminal region of the human IRAK4 protein. Species Sequence Homology: mouse and rat, 100%
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.
Extra Details:: MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. (IRAKs) are important mediators in the signal transduction of the Toll/IL-1 receptor (TIR) family. Members of the TIL family play a role in innate immune responses by coordinating host defense mechanisms. IRAK4 is the most recently discovered IRAK protein. IRAK-4 has been shown to interact with IRAK-1 and TRAF6 in an IL-1-dependent manner, and overexpression of IRAK-4 has been shown to activate NFkB as well as mitogen-activated protein (MAP) kinase pathways. Studies demonstrate that mice lacking IRAK4 are severely impaired in their IL-1, Toll-like receptor (TLR) signaling and response to viral and bacterial challenges; this indicates that IRAK4 has an important role in innate immunity. Humans lacking IRAK 4 exhibit pyogenic bacterial infections, while their blood and fibroblast cells fail to activate NF-kB. IRAK-4 is believed to be required for the LPS-induced activation of antigen-presenting cells. Applications: Suitable for use in Western Blot and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-3ug/ml Immunofluorescence: 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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