I8443-93
Interleukin 34 (IL-34, C16orf77, chromosome 16 open reading frame 77, hypothetical protein MGC34647)
Size
£889.00
- SKU:
- I8443-93
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Purified, NS0-derived, recombinant mouse IL-34, Asn21-Pro235, (rmIL-34)
- Format:
- Lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Interleukin-34, or IL-34 is a newly discovered interleukin. It was found in a massive screen of secreted proteins by Lin et al. at Five Prime Therapeutics, a company based on the Mission Bay campus of UCSF. IL-34 increases monocyte viablity and acts through its receptor colony-stimulating factor 1. The protein is 39kD in size and forms homodimers. Applications: Suitable for ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-mIL-34. ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect mouse IL-34. The detection limit for rmIL-34 is approximately 0.5ng/well. Optimal dilution determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice