I8443-60B
Interleukin 17E (IL-17E, IL25, IL-25, Precursor, interleukin 25, Interleukin-25) (PE)
Size
£780.00
- SKU:
- I8443-60B
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from cell culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Val17-Ala169 from mouse IL-17E expressed in E. coli. Species Sequence Homology: human; 76% and rat; 91%.
- Clone:
- 8H118
- Format:
- Supplied as a liquid in saline, pH 7.4, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions. Mouse IL-17E cDNA encodes a 169 amino acid (aa) precursor protein with a putative 16 aa signal peptide. Mouse IL-17E shares from 24% to 32% sequence identity with the other mouse IL-17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland, spinal cord, and trachea. IL-17E binds and activates the IL-17 B Receptor (IL-17B R; alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3, 4) which is expressed in the kidney and liver, and at lower levels in the brain, testis and other endocrine tissues. The expression of IL-17B R is up-regulated under inflammatory conditions. Ligation of IL-17E to IL-17B R induces activation of nuclear factor kappa-B and stimulates the production of the proinflamatory cytokine IL-8. IL-17 has also been found to promote the expression of the prototypical Th2 genes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice