I8443-60

Interleukin 17E (IL-17E, IL25, IL-25, Precursor, interleukin 25, Interleukin-25)

Size

£850.00

SKU:: I8443-60
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to Val17-Ala169 from mouse IL-17E expressed in E. coli. Species Sequence Homology: human; 76% and rat; 91%.
Clone:: 8H118
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions. Mouse IL-17E cDNA encodes a 169 amino acid (aa) precursor protein with a putative 16 aa signal peptide. Mouse IL-17E shares from 24% to 32% sequence identity with the other mouse IL-17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland, spinal cord, and trachea. IL-17E binds and activates the IL-17 B Receptor (IL-17B R; alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3, 4) which is expressed in the kidney and liver, and at lower levels in the brain, testis and other endocrine tissues. The expression of IL-17B R is up-regulated under inflammatory conditions. Ligation of IL-17E to IL-17B R induces activation of nuclear factor kappa-B and stimulates the production of the proinflamatory cytokine IL-8. IL-17 has also been found to promote the expression of the prototypical Th2 genes. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells in Tramp‑C1 mouse prostate cancer cell line fixed with paraformaldehyde and permeabilized with saponin. ELISA (Capture): 2-8ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice